Background CKD Patients on routine hemodialysis (HD) are prone to medical complications and conditions that are potentially detrimental to the quality of life (QoL), such as anemia, malnutrition, low body mass index (BMI), HD shift time, and HD adequacy measured by Kt/V. CKD patients undergoing routine HD mostly have lowered QoL and are at higher risk for malnutrition, inflammation, hospitalization, and mortality, compared to the general population. This study intends to find out whether there is a correlation between these factors and the quality of life of patients undergoing routine hemodialysis. Methods The design of this study is a cross-sectional analysis of observational data. Hemodialysis patients from general hospital Prof. dr. R. D. Kandou Manado for 3 months from August to October 2017 were included. Fifty-two patients meet the inclusion and exclusion criteria. The correlation between quality of life with anemia, serum albumin, BMI, adequacy of HD, using Pearson correlation test (if normality test fulfilled) or Spearman correlation test (if the normality test not fulfilled) and Independent Samples T-test to assess the quality of life with HD shift time. Results This study found no correlation between hemoglobin levels (p=0.244, r=-0.098), BMI (p=0.473, r=-0.010), HD timing (p=0.082) and quality of life of the patients, but a significant correlation between serum albumin (p=0.020, r=0.286), HD adequacy measured by Kt/V (p=0.030, r=0.257) and subjects’ quality of life. Conclusion This study showed that serum albumin and Kt/V values had a significant correlation with quality of life, while hemoglobin, BMI and dialysis shift time are not related to the quality of life.